DNA+Replication

Learning Outcomes:


 * Define: “Semi-conservative replication”
 * Link DNA replication to the processes of mitosis and meiosis
 * Identify the leading and lagging strands of DNA where the double helix has been separated
 * Discuss the role in DNA replication played by the following enzymes: helicase; gyrase; DNA polymerase III, DNA polymerase I, RNA primase; and DNA ligase.
 * Explain how “okazaki fragments” are formed
 * Memorise the process of DNA replication as a series of steps (like a step by step method)
 * Discuss the importance of DNA replication

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__Collaborative Notes__ __[]__

Semi-conservative replication: The normal process of DNA synthesis, in which the two original strands of the molecule separate, and each acts as a template on which a new complementary strand is laid down.

The Lagging Strand explanation Acronym    L. REPOST - this is read backwards. T: Three prime to five primeend  S: Series of short DNA sequences   O: Okazaki Fragments   P: Primer (RNA) is laid at various points on lagging strand   E: Extension of Primer by Pol III   R: RNA is Replaced with DNA (Pol I)    L: Ligase Joins all Okazaki Fragments Together    These are the key words that should be in your overall explanation of DNA replication     LES STAC (its french :P)    L: Leading/Lagging   E: Enzymes (all 5)   S: SYnthesise    S: Semiconservative replication   T: Template   A: Anti Parallel   C: Complimentary